Abstract:
Tylophora indica Syn. Tylophora asthmatica of the family Aselepiadaeeae grow
abundantly all over Bangladesh and India. In Bangladesh it is popularly known as
"Anthamul". It is an important medicinal plant of our country and is used for the
treatment of several diseases specially for the treatment of asthma, dysentery and
. 12-15 emesIs
A review on the phytochemical investigations on the herb revealed that along with
other compounds, quite a large number of alkaloidal eompounds2o
-
25 have been
isolated from its roots, stems and aerial parts. But in comparison to the work on its
stems and roots, little work has been done on its leaves. As root, stem and aerial parts
of the plant contained alkaloids, it is quite logical that its leaves should also contain
alkaloids amongst other compounds. Alkaloids being physiologically active
compounds, our main objective was to isolate, separate and purify alkaloidal
compounds from the leaves of anthamul and to determine the molecular architecture
of the isolated alkaloids along with other compounds isolated.
The herb Tindica was cultivated in the Club premises of the Bangladesh University
of engineering and Technology (BUET). The leaves were collected, dried in the shade
and grinded into powder for the purpose of the experiment. The powder obtained was
successively extracted with Pet. ether, EtOAc and MeaI-!.
Separation and fractionation followed by chromatographic analyses of the EtOAc
extract REA resulted into the isolation of the three pure compounds EA-2 EA-3 and
EA-4. Fractionation followed by purification of the various extracts enable us to
isolate the pure compound A5 from methanol extract, ME. All the four compounds
responded to the usual color reactions of alkaloids showing that they are alkaloidal in
nature.
all the basis of IR, IE-nmr, llC-11Illl"spectral analyses, the molecular formula of the
compound EA-2 was found as C29I-I480..'3C-nmr spectral data of the compound EA-2
was in full agreement with the published data of the stigmasterol31.Thus on the basis
of the chemical and spectral analyses, tentatively the following structure (20) may be
assigned for the compound EA-2 and it can be named as Stigmastan-5,22-di-ene-3(3-
01 in comparison with the established structure (19) of the compound Stigmastan-
7,22-di-ene-3(3-01. Thus the compound EA-2 is a sterol......................
