Phytochemical and microbial analysis of Andrographis peniculata

Abstract

Acanthaceae plants such as Andrographis peniculata is enriched with important biologically active compounds. From the profound interest of these compounds and their useful biological activities, the leaves along with the stems were extracted with ethanol. Then the ethanol extract was partitioned with n-hexane, ethyl acetate and 1-butanol, respectively. The partitioned extracts were subjected to various chromatographic techniques to isolate the pure compounds for characterization and determination of their biological activities. Seven compounds were characterized from the plant extract. Among the compounds stigmasterol, andrographolide, •-sitosterol, daucosterol were isolated from the n-hexane part. Three other compounds andrographanin, 14- deoxyandrographolide, 12-hydroxyneoandrographolide were isolated from the ethyl acetate part. All the structures of the compounds isolated from the plant are as follows: ---------------------------