Abstract:
The plant Asparagus racemosus of Aspargaceae family is used various diseases upset stomach
(dyspepsia), constipation, stomach spasm and stomach ulcers. It is also used for fluid retention,
pain, anxiety, diarrhea, bronchitis, dementia and diabetes. The plant root was enriched with
important biologically active compounds such as sarsasapogenin, adscendin (A, B), asparanin
(A, B, C). So the plant is selected for further investigation. The plant’s dried root was extracted
with various solvent such as methanol, dichloromethane and various solvent extract was
obtained. During this investigation four compounds were isolated from various plant extracts. All
the compounds were identified preliminary by chemical methods and then the structure
elucidations of the compounds were performed by various spectral methods (IR, 1H NMR, 13C
NMR and DEPT) and were confirmed in comparison with the result that is available in the
published literatures. In this project, three steroidal type compounds stigmasterol [(3S,8S,9S,
10R,13R,14S,17R)-17-[(E,2R,5S)-5-ethyl-6methylhept-3-en-2-yl]-10,13-dimethyl-
2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol] , β-sitosterol
[17-(5-Ethyl-6-methylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-
1H-cyclopenta[a]phenanthren-3-ol], Daucosterol [β-Sitosterol glucoside] and one triterpinoid
compound Lueol acetated [(3β)-lup-20(29)-en-3ol acetate] find out from the roots of the plant.
Stigmasterol and β-sitosterolshowed low anti- microbial activity against gram positive bacteria
(Bacillus cereus, Bacillus polymyxa) and gram negative bacteria (Klebsiella sp., Shigella
sonnei,)
Lupeol acetate also showed low anti microbial activity against gram positive (Bacillus cereus,
Bacillus subtilis, Mycobacterium tuberculosis) and gram negative Shigella sonnei.
The steroid Daucosterol showed moderate activity against Bacillus subtillis and gram negative
bacteria Pseudomonas.
Thus the isolation, characterization and investigation of anti microbial activity of the compounds
from the root of plant Asparagus resemosus might generate much synthetic and biological
interest in this field of the natural products.
